Figure 3.
Dynamitin N terminus disrupts Golgi organization but not dynactin structure. (A) Cos7 cells transfected with the highly conserved dynamitin N terminus (AA 1–87) were stained for dynamitin (pAb dynamitin; left) and the Golgi complex (giantin; right). (B) Lysates of Cos7 cells transfected with β-gal, dynamitin N terminus, or HA-dynamitin were analyzed by velocity sedimentation (Echeverri et al., 1996). Transfection efficiency was ≥50%. Gradient fractions were immunoblotted with antibodies to p150Glued, p62, and Arp1. Sedimentation standards are indicated.