Figure 8.

Effects of p150^Glued and CLIP-170 overexpression on CLIP-170 and dynactin localization. (a and b) p150^Glued overexpression. (c–f) Effects of wt CLIP-170 overexpression on p150^Glued (c and d) and Arp1 (e and f) staining. (g and h) Overexpression of CLIP-170ΔT (lacking the C-terminal domain that contains the metal-binding motif). HeLa cells were stained for CLIP-170 (pAb 55, a and e; 2D6 and 4D3, c; anti-myc mAb 9E10, g) or dynactin (p150^Glued: mAb 150.1, b; pAb 150^Glued, d and h; Arp1: mAb 45A, f). All were fixed with MeOH. Bars, 20 μm. Inset magnifications, 2×.