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. 2008 Aug 20;79(5):947–953. doi: 10.1095/biolreprod.108.070987

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© 2008 by the Society for the Study of Reproduction, Inc.

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FIG. 1. — The effect of GNRH pulse frequency on Lhb and Fshb PTs and Fst mRNA. The β subunit PTs and Fst mRNA levels were measured in pituitary RNA from male rats that had been pulsed with GNRH every 30 min or 240 min for 8 h. Rats were euthanized and pituitaries collected 5 min after the last pulse. All data are presented as percentage (±SEM) of controls (n = 6–7 per group). Means with different letters are significantly different (P < 0.05). Control means were: 47.0 ± 7.5 fg competitor RNA/100 ng RNA for Fshb PT, and 96.2 ± 10.5 fg competitor RNA/100 ng RNA for Lhb PT and 12.7 ± 1.0 fg plasmid/100 ng RNA for Fst mRNA.