Figure 7.
Cell delivery via CPC composite-hydrogel construct. (A) Cell-seeded alginate hydrogel beads. Cells were encapsulated into alginate beads which were then mixed into three pastes: conventional CPC, CPC-chitosan, and CPC-chitosan-mesh. (B) CPC paste mixed with the cell-seeded hydrogel beads. After 1-day culture inside the setting cements, there were numerous live cells and very few dead cells, indicating that the alginate beads adequately protected the cells. The cell viability (mean ± sd; n = 5) was measured using the Wst-1 assay. The absorbance at 450 nm was (1.36 ± 0.41) for the conventional CPC and (1.29 ± 0.24) for CPC-chitosan composite, similar to the (1.00 ± 0.14) for the control with the beads in the culture medium without any CPC (p > 0.1).