Figure 3.

Mutagenesis of the Rho3 effector domain. (A) Alignment of the 14 amino acids of the effector domains of Rho3, H-ras, and Cdc42. (B) Forty-six single-point mutations generated by oligo-directed mutagenesis. Growth at each temperature was recorded as follows: (+) = wild-type growth, (+/−) = borderline growth, (−/+) = slight growth above background, and (−) = no growth. (C) Growth of the rho3 mutants at the permissive and restrictive temperature. Plates at 25°C were incubated for 4 d, and plates at 14°C plates were incubated for 7 d.