Figure 6.

Electron microscopy of rho3-V51 cells reveals accumulation of post-Golgi vesicles and a conditional defect in vesicle delivery from the mother to the bud. (A) Three panels of rho3-V51 at both 25 and 14°C as well as single panels of RHO3 at 25 and 14°C are shown. Preparation of the cells is described in MATERIALS AND METHODS. (B) Cells were scored for the location of accumulated vesicles, and bar graphs show the percentage of cells with vesicles in the bud/neck region, in the mother cell, and in the entire cell. Approximately 30 cells were counted for rho3-V51 at each temperature, and 20 cells were counted for the RHO3 cells (wt). Only small budded cells were counted. Bars, 1 μm.