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HSF1 and HSF2 are present in mouse sperm nuclei. Protein extracts prepared from mouse sperm nuclei were separated on an 8% SDS polyacrylamide gel and assayed by Western blot using goat polyclonal antibodies against HSF1 (top panel) or HSF2 (bottom panel). Prestained protein markers were used to indicate molecular mass (kDa). Images are representative of results obtained from extracts prepared from at least two different sets of animals (at least two animals per group).
