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Aquaporin-2 (AQP2) phosphorylation in response to vasopressin. A: time course of change in AQP2 phosphorylation at S256 and S261 following 1 nM dDAVP. *Statistically significant. B: immunofluorescence confocal microscopy of inner medullary collecting ducts (IMCDs) double stained with antibodies against AQP2 pS256 (green) and AQP2 total (red). IMCDs were treated with vehicle (top) or 1 nM dDAVP (bottom) for 2 min. White asterisks indicate lumens of the IMCDs.
