Figure 2. Shark Myelin Po isoforms.
Western blot of purified shark myelin (5μg) showed several isoforms for Po. An antibody raised specifically to the shark cytoplasmic region (PoCy1) shows three bands for Po, while antibodies to the extracellular domain (PoEx) or a polyclonal antibody raised against purified Po (RabA) showed two strong bands and a fainter one. The antibody raised against the highly conserved cytoplasmic end of Po showed only one band. A total of 4ug of protein from purified myelin were loaded on each lane.