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. Author manuscript; available in PMC: 2008 Oct 28.
Published in final edited form as: Brain Behav Evol. 2008 Jul 17;72(1):48–58. doi: 10.1159/000145717

Figure 6. Developmental expression of myelin Po.

Figure 6

Shark brains from embryos at different developmental stages (4, 4.4 9 and 22cm embryos) were solubilized and ran on an SDS-PAGE gel. Western blot was done with the PoCy1 antibody that recognizes all three shark isoforms. Only the oldest embryo showed significant amounts of Po, and had three isoforms, the 32 and 28kD as well as a novel 34kD (arrowhead). The total protein loaded was: 16μg of protein for the 4, 4.4 and 9cm embryos, 4μg for the 22cm embryo and 2μg for the positive control SSM (shark spinal cord myelin).