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Still images taken from the supplemental movies (available online at www.biolreprod.org). Sperm from Ldhc+/- (HET) and Ldhc-/- (KO) mice were collected in capacitation medium and incubated 1 h or 4 h at 37°C in 5% CO2 in humidified air. Movies were taken with a Leica DFC420 digital camera mounted on a Leica DM IRB inverted phase microscope with a 20X lens and captured using Leica LAS software version 2.8.1.
