Table 2.
Percentage of in vivo fertilized eggs after natural mating with wild type (WT) or Ldhc-/- (KO) males.
A.
|
B.
|
||||
---|---|---|---|---|---|
genotype | Fertilized | 2-cell embryos | 4-cell embryos | Morula + blastocyst | Degenerated cells |
WT (A.179/B.229) | 62.77 % ±17.03 | 52.18 % ±23.65 | 46.93 % ±22.29 | 75.33 % ±12.38 | 24.67 % ±11.67 |
| |||||
KO (A.263/ B.259) | 0.30% ±0.81 | 3.41 % ±5.30 | 0 % | 0 % | 100 % |
| |||||
Control (195) | 0 % | 6.38 % ±6.08 | 0% |
Eggs were collected: (A) the day following mating; fertilization was assessed by the presence of pronuclei and development to two-cell and four-cell embryos; (B) 4.5 days after mating; number of in vivo-derived blastocysts were counted. Results are expressed in percentage of fertilized, two-cell, four-cell embryos or blastocysts from total eggs collected. Controls are eggs from superovulated non-mated females, and represent spontaneous divisions. The total numbers of eggs analyzed for (A) and (B) are indicated in parentheses. At least 3 independent experiments were performed. Values are means ±SEM.