Figure 4. Effect of duration of preincubation (0, 20, 40, and 60 min with inhibitor and NADPH regeneration system) on the extent of inhibition of CYP3A-mediated triazolam 1′-and 4-hydroxylation by 12.5μM (A) and 50μM (B) curcuminoid extract in human liver microsomes.
Assay details are provided in the text. Column heights correspond to the mean ± standard error of triplicate determinations using HLM from three different individuals.