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Acute fos mRNA response to nicotine in the CG (Δfos) was impaired by 6-OHDA pretreatment 5 days, but not 2 days, earlier. Δfos to nicotine per se in 6-OHDA pretreated rats was calculated by subtracting the chronic effect of 6-OHDA on fos (see Fig, 4B) from the total fos seen 30 min after nicotine stimulation. See the Results section for further details of the calculation (*significantly different from saline pretreatment).
