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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Autophagy. 2008 Jun 16;4(6):770–782. doi: 10.4161/auto.6458

Figure 5.

Figure 5

GFP-ERK2 granules colocalize with autophagosomes and lysosomes. (A) Representative confocal sections of 6-OHDA treated cells transiently co-expressing GFP vector or GFP-ERK2 and an N-terminal RFP fusion of LC3 (Cherry-LC3), or (B) co-expressing an N-terminal RFP fusion of ERK2 (RFP-ERK2) and GFP-LC3, or (C) expressing RFP-ERK2 and stained for endogenous LC3 by immunofluorescence with blue nuclear counterstain (scale bar: 20 μm). Arrows point to LC3 puncta colocalizing with ERK2 granules. (D) Representative confocal sections of LysoTracker Red (LTR) stained cells expressing with GFP vector control or the indicated N-terminal GFP fusion constructs of ERK2 (scale bar: 20 μm). Arrows point to GFP-ERK2 granules colocalizing with lysosomes (E) Summary quantification of the percent of GFP-ERK2 granules colocalizing with lysosomes per cell (means ± s.e.m. of n = 3–5 experiments with 25–30 cells each; ⊕:p < 0.05 vs GFP, ø: p < 0.05 vs GFP-ERK2-WT. (F) Summary quantification of the average number of lysosomes per cell (means ± s.e.m. of n = 3–5 experiments with 25–30 cells each; ø:p < 0.05 vs. GFP, ⊗:p < 0.05 vs. GFP-ERK2-WT).