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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Apr 30;326(2):532–545. doi: 10.1124/jpet.108.137372

Table 1.

Primer and Probe Sequences

Gene Oligonucleotide Conc., nM Sequence 5′-3′ MgCl2, mM
TNF-α Forward Primer 200 CTGTACCTTATCTACTCCCAGGTTCTC 2.5
Reverse Primer 200 TCTCCTGGTATGAAATGGCAAA
FAM-labeled Probe 100 TGCTCCTCACCCACACCGTCAGC
IL-1β Forward Primer 200 CTCGACAAGAGCTTCAG 3.5
Reverse Primer 250 CACGGGCAAGACATAG
FAM-labeled Probe 100 AAGGCAGTGTCACTCATTGTGGC
IL-6 Forward Primer 250 TCAACTCCATCTGCCCTTCAG 2.5
Reverse Primer 350 TGTCAACAACATCAGTCCCAAGA
CY5-labeled Probe 100 TCTCTCCGCAAGAGACTTCCAGCCAGTT
GR Forward Primer 300 AACATGTTAGGTGGGCGTCAA 5
Reverse Primer 300 GGTGTAAGTTTCTCAAGCCTAGTATCG
FAM-labeled Probe 100 TGATTGCAGCAGTGAAATGGGCAAAG