Table 1.
Gene | Oligonucleotide | Conc., nM | Sequence 5′-3′ | MgCl2, mM |
---|---|---|---|---|
TNF-α | Forward Primer | 200 | CTGTACCTTATCTACTCCCAGGTTCTC | 2.5 |
Reverse Primer | 200 | TCTCCTGGTATGAAATGGCAAA | ||
FAM-labeled Probe | 100 | TGCTCCTCACCCACACCGTCAGC | ||
IL-1β | Forward Primer | 200 | CTCGACAAGAGCTTCAG | 3.5 |
Reverse Primer | 250 | CACGGGCAAGACATAG | ||
FAM-labeled Probe | 100 | AAGGCAGTGTCACTCATTGTGGC | ||
IL-6 | Forward Primer | 250 | TCAACTCCATCTGCCCTTCAG | 2.5 |
Reverse Primer | 350 | TGTCAACAACATCAGTCCCAAGA | ||
CY5-labeled Probe | 100 | TCTCTCCGCAAGAGACTTCCAGCCAGTT | ||
GR | Forward Primer | 300 | AACATGTTAGGTGGGCGTCAA | 5 |
Reverse Primer | 300 | GGTGTAAGTTTCTCAAGCCTAGTATCG | ||
FAM-labeled Probe | 100 | TGATTGCAGCAGTGAAATGGGCAAAG |