Table 3.
Model Parameters for Glucocorticoid Receptor and Corticosterone Regulation.
| Parameter (units) | Description | Estimatea |
|---|---|---|
| α1 (ng_GR/ng_TNF) | Intrinsic activity of TNF-α mRNA on GR synthesis | 0.01010 |
| α2 (ng_GR/ng_IL-1β) | Intrinsic activity of IL-1β mRNA on GR synthesis | 0.0002894 |
| kin_GRm (hr−1) | Synthesis rate constant for GR mRNA | 102.5 |
| GRm0(ng/mg_RNA) | Glucocorticoid receptor mRNA baseline | 268.1b |
| IC50_GRm/DRN (nMol) | IC50 for CST effects on GR mRNA via GR | 545.7 |
| γDR | Hill coefficient of CST effects on GR mRNA | 10.00b |
| β1 (ng_CST·mL−1·ng_TNF−1) | Intrinsic activity of TNF-α mRNA on CST synthesis | 0.01118 |
| β2 (ng_CST·mL−1·ng_IL1β −1) | Intrinsic activity of IL-1β mRNA on CST synthesis | 0.00001159 |
| kin_CST (hr−1) | Synthesis rate constant for plasma CST | 1575 |
| CST0 (ng/mL) | Plasma CST baseline | 129.4b |
| ksyn_GR (nM hr−1 ng_GRmRNA−1) | Synthesis rate constant for glucocorticoid receptor | 0.1053 |
| ffGC·kon_C (hr−1) | Binding rate for steroid in plasma to receptor in tissue | 0.2530 |
| kRE_C (hr−1) | Release constant for CST & GR complex from nucleus | 85.04 |
| kt5 (hr−1) | Transduction constant for cytokine effects on GR mRNA | 0.002374 |
| kdgr_GR (hr−1) | Degradation rate constant for free glucocorticoid receptor | 0.054b |
| kT (hr−1) | Rate constant for DR complex translocation to the nucleus | 58.2b |
| RF | Fraction of DRN returned to free glucocorticoid receptor | 0.93b |
a
S-ADAPT Software was unable to generate CV%
b
Parameter value was fixed