Fig. 1. Production of VEGF, IL-6 and IL-8 as a function of tumor cell number.
H460 tumor cells were plated onto 96-well plate at 10×104, 7.5×104, 5×104, 2.5×104 and 1.25×104cells/well. After 24 h the supernatants were collected and concentrations of VEGF, IL-6, and IL-8 were determined using multiplexed immunobeads technology. Data presented as mean concentrations (pg/ml) ± SD.