Figure 1. Structures of various lesions used in this study.
DNA duplexes containing the lesion base OG, the hydantoin products, guanidinohydantoin (Gh) and the two diastereomers of spiroiminodihydantoin (Sp) derived from oxidation of OG, thymine glycol (Tg) and 5-hydroxycytosine (5-OHC) were used as substrates to evaluate the relative processing of these lesions by hNEIL1.