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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2008 Jul 6;15(8):842–848. doi: 10.1038/nsmb.1444

Figure 1.

Figure 1

Antisense transcription at the PR promoter. (a) Locations of PCR primers used for quantifying the presence of transcripts throughout the PR gene. (b) PCR detection of RNA at the PR promoter without addition of reverse transcriptase (No RT), RNA treated with reverse transcriptase (RT) and genomic DNA (gDNA). (c) Levels of RNA evaluated using qPCR from polyadenylated RNA purified from MCF7 cells. (d) Location of antisense transcripts within the PR promoter in T47D or MCF7 cells. Error shown is s.d., calculated from four independent isolations of poly-A RNA and duplicate qPCR analysis of each sample. PRA, transcription start site for the A isoform of PR. PRB, transcription start site for the B isoform of PR.