. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: Cell Signal. 2008 Jul 11;20(11):1968–1977. doi: 10.1016/j.cellsig.2008.07.007

Table 1. Kinetic parameters of PZA and mutants.

MyrArf1•GTP was titrated into reactions contain 0.4 nM of either [R407D]PZA or [H405D N406D]PZA and initial rates were determined as described in Luo et al. [15]. The Km and Vmax were estimated from a Michaelis-Menten plot and the kcat calculated from the Vmax. * data from Luo et al. [15].

Protein	K_m (μM)	k_cat (/sec)
PZA*	2.2±0.5	57.3±4.6
[R360Q]*	6.8±1.8	13.8±4.7
[R407D]	8.3±3.3	15.3±3.3
[H405D N406D]	9.7±3.6	15.9±3.4