Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Naunyn Schmiedebergs Arch Pharmacol. 2008 Jun 4;378(3):261–274. doi: 10.1007/s00210-008-0313-8

Fig. 4. Concentration/response curves for the effects of SDF-1α and AMD3100 on GTPase activity in Sf9 membranes expressing CXCR4.

Fig. 4

High-affinity GTPase activity was determined as described in Materials and Methods in Sf9 membrane 1182 (CXCR4 + Gαi2 + Gβ1γ2). A, Effects of SDF-1αand AMD3100 on basal GTPase activity. B, Inhibitory effect of AMD3100 on GTPase activity stimulated by SDF-1α (10 nM). Data shown are the means ± SD of a representative experiment performed in triplicates. Similar results were obtained in two independent experiments with different membrane preparations.