Table 5.

Comparison of protein levels and mRNA levels of Kcnb1 in mouse brain.

	MRNA Microarray analysis	Protein Western blot analysis
BXD 13	9.19±0.06 ****	1.96±0.13 **
BXD 38	9.95±0.05 ****	2.52±0.11 **
	Microarray analysis	Western blot
HAFT1	8.39±0.04 ***	1.01±0.07*
LAFT1	9.13±0.13 ***	1.22±0.07*

Western blot analysis of Kcnb1 protein was performed as described in the text. Densitometric measures were derived from images such as the ones illustrated in Figure 4. mRNA levels were obtained through analysis of Affymetrix MOE430V2 arrays. Data are mean ± SEM. There were 6 mice/strains used in Western blot and 4-6 mice/strain in microarray analysis. BXD 13 mice display high AFT while BXD 38 mice display low AFT. HAFT1/LAFT1 mice were selectively bred for high (HAFT) and low (LAFT) levels of AFT. t-test was used to compare expression values between BXD13 mice and BXD 38 mice and between HAFT1 mice and LAFT1 mice.

^*indicates p<0.05,

^**p <0.01,

^***p=0.002,

^****p=0.00002.