Fig. 1.

Representative results from a semiquantitative RT-PCR experiment showing induction of host gene expression patterns stimulated by T. vaginalis secreted proteins. Total RNA from MS-74 VECs incubated with growth medium alone or with secreted proteins was isolated (Experimental procedures). RNA was reverse-transcribed using oligo(dT) primer and PCR performed using gene-specific primers.

A. Showing the RT-PCR products separated on ethidium bromide (EtBr)-stained gels after electrophoresis in 2% agarose.

B. Illustrating the extent of gene expression pattern relative to the housekeeping GAPDH gene used as a control. The values were obtained by scanning the intensity of bands from pictures of the gels in A using the Scion image beta program. The expression for each gene was relative to baseline density for GAPDH plotted on the graph. This experiment was repeated on four separate occasions with similar results.