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. Author manuscript; available in PMC: 2008 Oct 28.
Published in final edited form as: J Am Chem Soc. 2008 Jun 4;130(26):8215–8222. doi: 10.1021/ja0774562

Figure 4.

Figure 4

(A) Experimental (black line) and simulated (red line) EPR spectra of resting Co-ImiS*; the simulation was calculated using ℋ = βg.B.S + S.D.S with spin Hamiltonian parameters of S =3/2, MS = ±½, g(x,y,z) = 2.29, 2.29, 2.50, D = 50 cm−1 and E/D = 0.09. Traces B – D show partial EPR spectra from Co(II) in Co-ImiS*. (B) Resting Co-ImiS*. (C) Co-ImiS* after reaction with 1 mM imipenem for 12 ms at 2 °C. (D) Co-ImiS* after reaction with 1 mM imipenem for 1 min at 2–25 °C. The insets in C and D show the 59Co hyperfine region (× 4 in each case). Spectra were recorded at 9.634 GHz, 12 K, 1 mW microwave power, with 1.4 mT (14 G) magnetic field modulation at 100 kHz.