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. Author manuscript; available in PMC: 2008 Oct 28.
Published in final edited form as: J Cell Biochem. 2008 Oct 15;105(3):859–865. doi: 10.1002/jcb.21881

Fig. 2.

Fig. 2

The second leucine zipper within the FIAT sequence is responsible for repressing ATF4-induced transcriptional activity. Transient transfection assays in MC3T3-E1 osteoblastic cells with expression vectors for ATF4 and wild-type or mutant FIAT. A: Transfection with wild-type (WT) or ZIP2 DEL FIAT. B: Transfection with WT or the site-specific M1, M2, or M3 mutants. The expression of the recombinant proteins was monitored by Western blotting at the bottom of each panel. The M2 mutant reproducibly exhibited faster migration than WT FIAT or the M1 and M3 mutants in SDS-PAGE. Mutation within the second leucine zipper of FIAT rendered FIAT unable to inhibit ATF4-mediated gene transcription. **P<0.01. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]