Table 3.

Activities of NOS (pmol/min/mg protein) and GTP-CH (pmol/h/mg protein), as well as concentrations of tetrahydrobiopterin (pmol/g tissue) and NADPH (nmol/g tissue) in tissues of young pigs fed diets supplemented with cholesterol and DHA¹.

	Control	Cholesterol	DHA	Cholesterol+ DHA	P value (Chol)	P value (DHA)	P value (Interaction)
Brain
NOS	162±10	203±13	235±14	259±14	0.026	0.0001	0.515
GTP-CH	4.88±0.21	5.71±0.16	6.30±0.20	6.65±0.24	0.014	0.0001	0.258
BH4	464±15	518±11	632±15	634±21	0.103	0.0001	0.133
NADPH	24±1.1	25±1.0	33±1.0	32±1.3	0.907	0.0001	0.0001
Liver
GTP-CH	70±3.9	71±5.0	87±1.6	86±3.4	0.908	0.001	0.892
BH4	3629±283	3747±294	4850±209	4840±239	0.838	0.001	0.809
NADPH	282±15	286±14	370±18	375±18	0.778	0.001	1.00
Muscle
NOS	21±1.3	21±1.4	31±1.2	32±1.4	0.440	0.0001	0.630
GTP-CH	0.99±0.06	0.98±0.07	1.44±0.08	1.39±0.07	0.740	0.0001	0.765
BH4	75±5	78±2	115±10	115±10	0.856	0.0001	0.856
NADPH	67±3.4	64±3.0	85±3.5	80±4.6	0.309	0.0001	0.814

¹Values represent means ± SEM, n = 4.