Table 1.
Constructs and clones used in the study.
| Construct or clone | Description | Source |
|---|---|---|
| pBBE22 | pBSV2 carrying a bbe22 copy | (Purser et al., 2003) |
| pBBE22-ospC′ | pBBE22 carrying promoterless ospC fused with flaB promoter | (Xu et al., 2006) |
| pBBE22-ospA′ | pBBE22 carrying promoterless ospA fused with flaB promoter | This study |
| pBBE22-ospE′ | pBBE22 carrying promoterless ospE fused with flaB promoter | This study |
| pBBE22-vlsE′ | pBBE22 carrying promoterless vlsE fused with flaB promoter | This study |
| pBBE22-dbpA′ | pBBE22 carrying promoterless dbpA fused with flaB promoter | (Xu et al., 2007b) |
| pBBE22-CpospA | pBBE22 carrying promoterless ospA fused with ospC regulatory elementsa | This study |
| ΔospC | ospC mutant | (Xu et al., 2007a) |
| ΔospA | ospA mutant | This study |
| ΔospC/E22/1 | ospC mutant without increased osp expression | This study |
| ΔospC/E22/2 | ospC mutant without increased osp expression | This study |
| ΔospC/ospC′/1 | ospC mutant expressing ospC driven by flaB promoter | This study |
| ΔospC/ospC′/2 | ospC mutant expressing ospC driven by flaB promoter | This study |
| ΔospC/ospA′/1 | ospC mutant expressing ospA driven by flaB promoter | This study |
| ΔospC/ospA′/2 | ospC mutant expressing ospA driven by flaB promoter | This study |
| ΔospC/ospE′/1 | ospC mutant expressing ospE driven by flaB promoter | This study |
| ΔospC/ospE′/2 | ospC mutant expressing ospE driven by flaB promoter | This study |
| ΔospC/vlsE′/1 | ospC mutant expressing vlsE driven by flaB promoter | This study |
| ΔospC/vlsE′/2 | ospC mutant expressing vlsE driven by flaB promoter | This study |
| ΔospC/dbpA′/1 | ospC mutant expressing dbpA driven by flaB promoter | This study |
| ΔospC/dbpA′/2 | ospC mutant expressing dbpA driven by flaB promoter | This study |
| ΔospC/FL/1 | ospC mutant expressing ospC controlled by ospC regulatory elements | (Xu et al., 2007a) |
| ΔospC/FL/2 | ospC mutant expressing ospC controlled by ospC regulatory elements | (Xu et al., 2007a) |
| ΔospC/CpospA/1 | ospC mutant expressing ospA controlled by ospC regulatory elements | This study |
| ΔospC/CpospA/2 | ospC mutant expressing ospA controlled by ospC regulatory elements | This study |
| ΔospA/CpospA/1 | ospA mutant expressing ospA controlled by ospC regulatory elements | This study |
| ΔospA/CpospA/2 | ospA mutant expressing ospA controlled by ospC regulatory elements | This study |
a
The ospC regulatory elements include both operator and promoter.