Fig. 8.
CES1 protein levels are not down-regulated in THP-1 monocytes following exposure to paraoxon. THP-1 cells were incubated with either vehicle (ethanol) or paraoxon (10 μM) for 24 h. Cells were collected, washed, and lysed. Cell lysate proteins were separated by SDS-PAGE and proteins transferred to a PVDF membrane, which was then probed with anti-CES1 and anti-β-actin antibodies. Bar graph represents the pixel density of each gel band (mean ± SD, n = 3).