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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Pharmacol. 2008 May 5;74(2):392–402. doi: 10.1124/mol.108.046946

Figure 2.

Figure 2

Effect of actinomycin D and cycloheximide on Compound-induced TNF-α production by J774.A1 macrophages. J774.A1 macrophages were pretreated with actinomycin D (ActD; 1 µg/ml), cycloheximide (CHX; 5 µg/ml), or control DMSO for 30 min, followed by addition of 50 µM Compound 1 (open bars) or 2 (solid bars) for 12 hr. TNF-α was measured in the cell supernatants by ELISA. The data are presented as the mean±SD of triplicate samples from one experiment, which is representative of three independent experiments.