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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Mol Pharmacol. 2008 Jun 26;74(3):685–696. doi: 10.1124/mol.108.048066

Figure 10.

Figure 10

Effect of pretreating with CP-532,903 on chemotaxis of Percoll gradient purified mouse bone marrow neutrophils towards various chemoattractants. Neutrophils were incubated (37° C) with vehicle or CP-532,903 (100 nM) for 30 min in the presence of ADA (1 unit/mL) with or without rm TNF-α(100 ng/mL), prior to addition to the upper wells of trans-well assays containing increasing concentrations of C5a (A and B), IL-8 (C and D), or PAF (E and F) in lower wells. The chemotaxis index was calculated as described in Methods after allowing the cells to migrate for 35 min. Results are presented as the mean ± SEM. p < 0.05 versus the vehicle-treated group by two-way ANOVA, n = 3–6.