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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Mol Pharmacol. 2008 Jun 26;74(3):685–696. doi: 10.1124/mol.108.048066

Figure 4.

Figure 4

Superoxide production by Percoll gradient purified mouse bone marrow neutrophils in response to fMLP (1 μM). Studies were conducted with naïve neutrophils, neutrophils primed with TNF-α (30 min incubation with 100 ng/mL rm TNF-α), or treated with superoxide dismutase (SOD). Superoxide production was measured using the chemiluminescent probe MCLA. Relative light units (RLUs) were obtained for 3 minutes at 10 second intervals. The inset depicts the cumulative relative light units generated over 3 minutes after subtracting baseline values. Results are presented as the mean ± SEM. *, p < 0.05 versus the unprimed fMLP-induced group by one-way ANOVA followed by a Student’s t test, n = 3.