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Binding of cellular proteins to Hsp90α immobilized on magnetic beads. Hsp90α (NT)-MBs were incubated with lysates from KU-812 basophiles. The nonadsorbed material and the material eluted from the beads were analyzed by Western blot with antibodies against p60Hop (top panel) or the Hsp90 cochaperone, p23 (bottom panel). Lane 1, control; lane 2, eluted material from control; lane 3: Hsp90α-NT supernatant; lane 4, eluted material from Hsp90α-NT.
