. Author manuscript; available in PMC: 2008 Oct 28.

Published in final edited form as: Anal Chem. 2008 Aug 12;80(19):7571–7575. doi: 10.1021/ac801153h

Table 1.

Binding Profile of Recombinant Proteins Added Singly onto Hsp90α Immobilized on Magnetic Beads^a

	control(NT)	Hsp90α(NT)-MBs
	nonadsorbed	nonadsorbed	eluted
eNOS	100	8 ± 1	94 ± 44
p60Hop	100	14 ± 23	64 ± 22
Hsp70	100	70 ± 29	18 ± 19

Control(NT) and Hsp90α(NT)-MBs were incubated with individual recombinant proteins as indicated in Materials and Methods. Nonadsorbed and eluted materials were resolved by SDS-PAGE and analyzed by Western blot. The protein bands were visualized with Hyperfilms and quantitated by densitometry. Values are means ± standard deviation from three to four independent experiments. The signal associated with nonadsorbed material from control(NT) beads was arbitrarily given the value of 100%.