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. Author manuscript; available in PMC: 2008 Oct 29.
Published in final edited form as: J Hepatol. 2008 Jun 9;49(3):373–383. doi: 10.1016/j.jhep.2008.05.015

Figure 3. shRNA-mediated knockdown of WWOX in SNU449 cells inhibits staurosporine-induced apoptosis and enhances FGF2-induced cell proliferation.

Figure 3

(A) Relative proliferation of vector and WWOX shRNA transfected SNU449 cells assessed by MTT assay. Transient transfection of WWOX shRNA increased proliferation of SNU449 cells. Staurosoporine substantially inhibited proliferation of both vector and shRNA transfected cells. (B and C) WWOX shRNA decreased the sensitivity of SNU449 cells to staurosporine-induced apoptosis, as measured by DAPI staining and fluorescence microscopy. (D) SNU449 cells stably transfected with WWOX shRNA have approximately 40% lower WWOX mRNA expression than pSSH1p Vector control cells by real-time PCR; this result was confirmed by Western immunoblotting (inset). (E) Stable knockdown of WWOX increased proliferation of SNU449 cells. (F) Suppression of WWOX expression significantly enhanced FGF-mediated proliferation of SNU449 cells, as measured by BrdU incorporation.