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. 1999 Dec;10(12):4163–4176. doi: 10.1091/mbc.10.12.4163

Figure 7.

Figure 7

Ultrathin cryosections of VAMP-TAg/N49A PC12 cells. Internalized VAMP-TAg/KT3 antibody complexes were labeled on the sections with rabbit anti-mouse IgG and protein A-gold. Cells were either directly fixed after 40 min at 15°C (A) or first washed to remove plasma membrane-bound antibodies and then fixed (B and C) or first warmed to 37°C before fixation (D and E). After 40 min at 15°C, internalized VAMP-TAg was found in clathrin-coated endocytic vesicles (A, arrows) and the vacuolar domain of EEs (B and C). EE-associated tubules and vesicles were not labeled (B and C, arrowheads). Warming for 5 min to 37°C resulted in the appearance of internalized VAMP-TAg in EE-associated tubules and vesicles (D and E, arrowheads). PM, plasma membrane. Bars, 100 nm.