Table 2.
Summary of Dimers Between Intact Globin Chains for in Vitro Samples (RBCs Incubated with DCVCS 0.9–450 μM)
| DCVCS as cross-linker without additional monoadducts | DCVCS as cross-linker plus following monoadducts | |||
|---|---|---|---|---|
| DCVCS | DCVCS-GSH | DCVCS-Cys | ||
| DCVCS 0.9 μM | ||||
| Masstheo (Da) | 31204 | 32064 | - | - |
| Massobs (Da) | 31207 | 32065 | ||
| Cross-links | α1β1+1cl | β1β2+1cl+1 | ||
| DCVCS 4.5 μM | ||||
| Masstheo (Da) | 31204 | 31138; 31759 | - | - |
| Massobs (Da) | 31205 | 31140; 31758 | ||
| Cross-links | α1β1+1cl | α1α2+2cl+2; α2β2+1cl+3 | ||
| DCVCS 9 μM | ||||
| Masstheo (Da) | 31204 | - | 31843; 32616 | 30992 |
| Massobs (Da) | 31206 | 31840; 32620 | 30995 | |
| Cross-links | α1β1+1cl | α1β2+2cl+1; α1β2+1cl+3 | α1α1+2cl+1 | |
| DCVCS 90 μM | ||||
| Masstheo (Da) | - | 31759; 31138; 31745 | 31785 | 31657 |
| Massobs (Da) | 31762; 31138; 31744 | 31785 | 31662 | |
| Cross-links | α2β2+1cl+3; α1α2+2cl+2; 2β1+1cl+3 | α2β1+2cl+1 | α1β2+2cl+1 | |
| DCVCS 450 μM | ||||
| Masstheo (Da) | 31204 | 31759; 31138 | - | 31657 |
| Massobs (Da) | 31206 | 31762; 31138 | 31660 | |
| Cross-links | α1β1+1cl | α2β2+1cl+3; α1α2+2cl+2 | α1β2+2cl+1 | |
a
1cl (+159 Da) = 1 DCVCS cross-linker (between chains), 2cl (+159*2 Da) = 2 DCVCS cross-linkers, 3cl (+159*3 Da) = 3 DCVCS cross-linkers. Cross-links with and without monoadducts are shown. Monoadducts include: DCVCS (+195 Da; +195*2 Da; +195*3 Da), DCVCS-GSH (+466 Da, with up to 3 adducts as described above for DCVCS), DCVCS-Cys (+280 Da, with up to 3 adducts as described above for DCVCS). Example: αβ+1cl+2 defines a cross-link between the stated chains with 1 cross-linker (DCVCS) and 2 monoadducts.