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. Author manuscript; available in PMC: 2008 Oct 29.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Jun 5;233(9):1149–1160. doi: 10.3181/0802-RM-59

Figure 1. ABC-transporter gene expression and transporter specific efflux function in MDR-1 and MRP overexpressing cell lines.

Figure 1

The MCF7, NCI(ADR) and PANC-1 cells were used as controls for MDR-1 (P-gp) and MRP (MRP-1, to MRP-5) gene expression and MDR-1 and MRP mediated efflux function. In (A), the RT-PCR amplified products are shown in a representative gel picture. The densitometric values of product intensities (n=4) were normalized to β-actin levels. In (B), the ratios are presented as empirical densitometric units (D.U.). Drug-efflux data from calcein assays are shown in (C) and (D). Fluorescence readings (Fluorescence/mg of protein) observed in the absence (clear bars) or presence of either verapamil (C) or MK-571 (D) are shown (n=5). Error bars represent the ±SEM of values and significant differences in mRNA expression and calcein-retention, are indicated by P values (*, p<0/05; **, p<0.02).