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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Cell Biochem. 2008 Oct 1;105(2):585–595. doi: 10.1002/jcb.21859

Fig. 4.

Fig. 4

Iron chelation effect of quercetin is not involved in Her-2/neu down-regulation. A: SK-Br3 cells were pretreated with various amount of FeSO4 for 30 min and then 200 μM of quercetin was applied for additional 24 h. Cellular Her-2/neu protein level was compared by Western blot analysis. B: SK-Br3 cells were treated with 200 μM of desferrioxamine (DFX) for the indicated time. Cellular Her-2/neu protein level was compared by Western blot analysis. Accumulation of HIF-1α is shown as confirmation of the biological effect of DFX. C: SK-Br3 cells were treated with 200 μM of quercetin or 200 μM of FeSO4 for 4 h. For the samples with combined treatment, FeSO4 was applied 30 min before (Fe + Q) or after (Q + Fe) the treatment with quercetin. D: SK-Br3 cells were treated with quercetin (200 μM), DFX (200 μM), or both for 4 h. After Her-2/neu protein was immunoprecipitated, ubiquitination level and interacting amount of Hsp90 was compared by Western blot analysis.