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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Protein Expr Purif. 2008 Jul 17;62(1):104–110. doi: 10.1016/j.pep.2008.07.001

Figure 2.

Figure 2

SDS-PAGE for the purification of bovine DHFR. Lane 1: Protein Marker (Invitrogen, SeeBlue Plus2); lane 2, whole cell lysate, lane 3: digest with Sumo protease (Ulp1) at 4°C for 1 hour, lane 4: elution from Ni-NTA 5ml HisTrap, lanes 5 and 6: flow through by using Ni-NTA His tag second time.