Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Protein Expr Purif. 2008 Jul 17;62(1):104–110. doi: 10.1016/j.pep.2008.07.001

Table 3.

Oligonucleotides used to incorporate the 19 residue changes in the protein sequence of murine and bovine DHFR as shown in Table 2

1. AAG(K)33CAA(Q)
2. ACC(T)41GTG(V)
3. CGA(R)85AAA(K)
4. AGA (R)99 GAA (E)
5. CAA (Q) 103 GAC (D)
6. GCA (A) 107 ACA (T)
7. AGA (S) 108 AAT (N)
8. ATG (M) 112 GTT (V)
9. CAG (Q) 123 AAA (K)
10. CAA (Q) 128 AAA (K)
11. CTC (L) 132 GTT (V)
12. ACG (T) 147 GCA (A)
13. TTG (L) 154 TTC(F)
14. GGG (G) 155 GAG (E)
15. CTC (L) 167 CCG (P)
16. TCT (S) 168 CTC (L)
17. GAG (E) 169 GAC (D)
18. AAA (K) 186 AAC (N)
19. GAC (D) 187 AAC (N)