. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: Protein Expr Purif. 2008 Jul 17;62(1):104–110. doi: 10.1016/j.pep.2008.07.001

Table 5.

Kinetic characterization of bDHFR

Enzyme	Km DHFA µM	Km NADPH µM	kcat s⁻¹	Catalytic efficiency s⁻¹ µM⁻¹
bDHFR	0.35 ± 0.13	3.2 ± 0.6	24 ± 7	68
mDHFR	0.32 ± 0. 05	2.4 ± 0.5	29 ± 5	92
hDHFR	2.7 ± 0.5	5.7 ± 0.9	40 ± 2	15

All assays were conducted at 37°C with KCl in the assay, as described in methods.

b = bovine DHFR; m = murine DHFR; h = human DHFR.