Table 1.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pEAH47 | pEVS107 with 1.6 kb VFA1019 - sypA (VFA1020) intergenic region fused to lacZ | This work |
| pEVS107 | Delivery vector for Tn7::erm | (McCann et al., 2003) |
| pKG9 | pEVS79 (Stabb and Ruby, 2002) with the rscS locus from ~1 kb upstream to ~1 kb downstream of the gene but with an internal EcoRI/ClaI fragment carrying rscS removed | This work |
| pKG11 | pKV69 containing PrscS-rscS1 | (Yip et al., 2006) |
| pKG12 | pKV69 containing PrscS-rscS1, opposite orientation relative to PlacZ | This work |
| pKG13 | pKV69 containing PrscS-rscS2 | (Yip et al., 2006) |
| pKG32 | pKG11 with PrscS -35 element mutation | This work |
| pKG63 | pKV69 with rscS1 under control of PlacZ | This work |
| pKV69 | Mobilizable vector, CmR, TetR | (Visick and Skoufos, 2001) |
| pKV251 | pKV69 with RBS1 rscS under control of PlacZ | This work |
| pKV254 | pKV69 with rscS2 under control of PlacZ | This work |
| pKV255 | pKV69 with Leu25 rscS under control of PlacZ | This work |
| pKV256 | pKV69 with wild type rscS under control of PlacZ | This work |
| pKV283 | pKG11 deleted for the lac promoter | This work |
| pLMS33 | pKV69 containing rscS coding sequences plus 389 bp of sequence upstream of the ATG start codon, including PrscS- rscS (located 44–70 bp upstream of the ATG start codon) | (Visick and Skoufos, 2001) |
| Strains | Description | Reference |
| ES114 | V. fischeri wild type | (Boettcher and Ruby, 1990) |
| KV2566 | ES114 att Tn7::PsypA::lacZ | This work |
| KV3378 | ES114 ΔrscS | This work |