Effect of lidocaine TB on biophysical properties of V232I and L1308F. A, I-V relationship shows similar block by 30 μmol/L lidocaine at 0.06 Hz for V232I and L1308F (25.6±8.9% versus 21.8±2.9%, respectively). B, Steady-state inactivation curves of V232I and L1308F under 30 μmol/L lidocaine. Hinf50 (mV)=-91.92±0.76 (V232I in control), -102.21±0.82 (V232I with 30 μmol/L lidocaine), -96.28±1.36 (L1308F in control), -104.32±1.3 (L1308F with 30 μmol/L lidocaine).