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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Cell Biochem. 2008 Sep 1;105(1):167–175. doi: 10.1002/jcb.21809

Figure 4.

Figure 4

PAK5 is unique among group II PAKs for interaction with Raf-1 and does not bind to other Raf proteins. (A) HEK293 cells were co-transfected with Flag-tagged wt Raf-1 and the Myc-tagged PAKs shown. After serum starvation the PAKs were immunoprecipitated and tested for co-precipitation of Raf-1 by Western blotting. (B) HEK293 cells were transfected with Myc-tagged wt PAK5 and Flag-tagged wt Raf-1, A-Raf, or B-Raf. The PAK5 was immunoprecipitated and the co-precipitation of Raf proteins was tested by Western blotting using Raf isoform specific antibodies. The upper panels are Western blots for co-precipitated Raf isoforms, the middle panels are Western blots for immunoprecipitated PAK5, and the bottom panels are Western blots for Raf isoforms in the cell lysates. (C) Endogenous Raf-1 or A-Raf were immunoprecipitated from a rat brain homogenate and tested for the co-precipitation of endogenous PAK5 by Western blotting. Shown are representative experiments from three independent experiments.