Fig. 3.

Plots of (a) average relative Bcl-2 content; (b) average relative caspase-3 content; and (c) the product of average relative caspase-3 content and average relative Bcl-2 content versus 1/[EC₅₀ (NCS)] for cell death induced by neocarzinostatin for cell lines described in Table 1. Relative protein content was determined for caspase-3 and Bcl-2, respectively, by optical densitometry of Western blot bands, as described in Materials and Methods, in two independent experiments. In each panel, plots are shown for the average of the two values obtained for each cell line. R² was determined by linear regression; p was determined by two-way ANOVA.