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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Int J Cancer. 2008 Dec 1;123(11):2566–2573. doi: 10.1002/ijc.23898

Figure 1.

Figure 1

Immunohistochemical staining of lymphatic vessels and VEGF C in human head and neck tumor specimens. Formalin-fixed, paraffin-embedded 5 micron sections of 65 head and neck tumors were subjected to immunostaining, applying D2-40 monoclonal (A-C) and anti-VEGF C (G-I) polyclonal antibodies, as described under ‘Materials and Methods’. Shown are representative photomicrographs of specimens depicting low (A; +1) and high (B; +2) number of lymphatic vessels. Lymphatic vessel (arrow) adjacent to D2-40-positive tumor cells is shown in C. Shown are also photomicrographs of VEGF C negative (G), and positively stained specimens scored as weak (H; +1) and strong (I; +2) intensity. D-F. Double immunofluorescent staining. Head and neck tumor specimen was stained with anti heparanase polyclonal (green, D) and D2-40 monoclonal (red, E) antibodies, illustrating heparanase-positive tumor cells inside a lymphatic vessel lumen (merge, F), penetrating the lymphatic endothelium (arrow). Original magnifications: A,B × 20; D-F × 100; C, G-I × 40