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. 1999 Dec;10(12):4177–4190. doi: 10.1091/mbc.10.12.4177

Figure 2.

Figure 2

Effects of matrix adhesion or TPA treatment on GFP-fascin localization in LLC-PK1 cells. Cells adherent on laminin-1 for 1 h were double-stained with GFP-fascin (A) or antibody to β-actin (B). (C–E) GFP-fascin visualized after 1 h on fibronectin (C), in long-term adherent cells (D), or after treatment with 50 nM TPA for 1 h (E). Large arrows in A and B indicate coincidence of fascin with microfilaments, and small arrows indicate examples of coincidence in membrane ruffles. Bar, 5 μm for A and B, 10 μm for other panels.