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Expression of PKC family members in C2C12 cells and effect of matrix adhesion on PKCα localization. (A) Western blots of brain extract (lanes1, 3, and 6) and C2C12 whole cell extract (lanes 2, 4, and 7) were probed with antibodies to PKCα, PKCγ, and PKCλ. (B and C) C2C12 cells adherent for 1 h on TSP-1 (B) or fibronectin (FN) (C) were stained with antibody to PKCα. Arrows indicate regions of microspike formation in TSP-1–adherent cells that do not stain for PKCα. Bar, 8 μm.
