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. 1999 Dec;10(12):4177–4190. doi: 10.1091/mbc.10.12.4177

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Copyright © 1999, The American Society for Cell Biology

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Requirement for serine 39 in fibronectin-dependent phosphorylation of fascin. (A) Western blot of LLC-PK1 lines probed with FAS-C serum. (Lane 1) Parental cells; (lane 2) GFP-fascin overexpressors; (lane 3) GFP-fascin S39A overexpressors; (lane 4) GFP-fascin S39D overexpressors. (B–D) Western blots of two-dimensional IEF/SDS-PAGE gels of LLC-PK1 whole cell extracts probed with FAS-C serum. (B) LLC-PK1 GFP-fascin overexpressors after 1 h on laminin-1. (C) LLC-PK1 GFP-fascin overexpressors after 1 h on fibronectin. (D) LLC-PK1 GFP-fascin S39A overexpressors after 1 h on fibronectin.